Anti-Vimentin antibody[STJ98448]
Product nameAnti-Vimentin antibodyShort DescriptionMouse monoclonal against VimentinDescriptionMouse monoclonal to Vimentin.A
Product nameAnti-Vimentin antibodyShort DescriptionMouse monoclonal against VimentinDescriptionMouse monoclonal to Vimentin.A
| Product name | Anti-Vimentin antibody |
|---|---|
| Short Description | Mouse monoclonal against Vimentin |
| Description | Mouse monoclonal to Vimentin. |
| Applications | ELISA, IHC, WB |
| Dilution range | WB 1:500-1:2000 IHC 1:200-1:1000 ELISA 1:10000 |
| Specificity | Vimentin Monoclonal Antibody detects endogenous levels of Vimentin protein. |
| Protein Name | Vimentin |
| Immunogen | Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli. |
| Immunogen Region | 2-466aa |
| Storage Instruction | Store at -20°C, and avoid repeat freeze-thaw cycles. |
| Note | For Research Use Only (RUO). |
| Validated Application | ](/uploads/ueditor/20200413/1-20041314130Y52.png "Anti-Vimentin antibody[STJ98448](图1)"){kind=small} ](/uploads/ueditor/20200413/1-200413141315259.png "Anti-Vimentin antibody[STJ98448](图2)"){kind=small} |
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone ID | 4F2E9 |
| Reactivity | Human, Simian |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Isotype | IgGMale |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 7431 |
|---|---|
| Gene Symbol | VIM |
| Database Links | HGNC:12692 OMIM:116300 |
| Alternative Names | Vimentin |
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
| Post-translational Modifications | Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin . One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| Cellular Localization | Cytoplasm |
| Tissue Specificity | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
| Swiss-Prot Key | P08670_HUMAN |
](/uploads/ueditor/20200825/1-200R516032OY.png "Anti-Vimentin antibody[STJ98448](图3)")
