Anti-N-cadherin antibody[STJ94353]
Product nameAnti-N-cadherin antibodyShort DescriptionRabbit polyclonal against N-cadherinDescriptionN-cadherin is a protein e
Product nameAnti-N-cadherin antibodyShort DescriptionRabbit polyclonal against N-cadherinDescriptionN-cadherin is a protein e
| Product name | Anti-N-cadherin antibody |
|---|---|
| Short Description | Rabbit polyclonal against N-cadherin |
| Description | N-cadherin is a protein encoded by the CDH2 gene which is approximately 99,8 kDa. N-cadherin is localised to the cell membrane. It is involved in cell junction organization, ERK signalling, blood-brain barrier and immune cell transmigration. It is a calcium-dependent cell adhesion protein. They interact with themselves in a homophilic manner in connecting cells which contribute to the sorting of heterogeneous cell types. It also acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone. N-cadherin is expressed in the nervous system, liver, muscle, heart and eye. Mutations in the CDH2 gene may result in malignant pleural mesothelioma and hypoplastic left heart syndrome. STJ94353 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of N-cadherin protein. |
| Applications | ELISA, IF, IHC, WB |
| Dilution range | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:10000 |
| Specificity | N-cadherin Polyclonal Antibody detects endogenous levels of N-cadherin protein. |
| Protein Name | Cadherin-2 CDw325 Neural cadherin N-cadherin CD antigen CD325 |
| Immunogen | Synthesized peptide derived from human N-cadherin |
| Immunogen Region | 690-770 aa, C-terminal |
| Storage Instruction | Store at -20°C, and avoid repeat freeze-thaw cycles. |
| Note | For Research Use Only (RUO). |
| Validated Application | ](https://www.stjohnslabs.com/media/application_icon/IF.png "Anti-N-cadherin antibody[STJ94353](图1)"){kind=icon} ](https://www.stjohnslabs.com/media/application_icon/IHC.png "Anti-N-cadherin antibody[STJ94353](图2)"){kind=icon} ](https://www.stjohnslabs.com/media/application_icon/WB.png "Anti-N-cadherin antibody[STJ94353](图3)"){kind=icon} |
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Isotype | IgG |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene ID | 1000 |
|---|---|
| Gene Symbol | CDH2 |
| Molecular Weight | 130 kDa |
| Database Links | HGNC:1759 OMIM:114020 |
| Alternative Names | Cadherin-2 CDw325 Neural cadherin N-cadherin CD antigen CD325 |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density . |
| Post-translational Modifications | Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence . May be phosphorylated by OBSCN. |
| Cellular Localization | Cell membrane Cell membrane, sarcolemma Cell junction Cell surface. Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. |
| Swiss-Prot Key | P19022_HUMAN |
](/uploads/ueditor/20200817/1-200QG231494Z.png "Anti-N-cadherin antibody[STJ94353](图4)")
