Anti-Active Caspase-3 antibody[STJ97448]
Product nameAnti-Active Caspase-3 antibodyShort DescriptionMouse monoclonal against Active Caspase-3DescriptionActive caspase
Product nameAnti-Active Caspase-3 antibodyShort DescriptionMouse monoclonal against Active Caspase-3DescriptionActive caspase
| Product name | Anti-Active Caspase-3 antibody[STJ97448] |
|---|---|
| Short Description | Mouse monoclonal against Active Caspase-3 |
| Description | Active caspase-3 is a protein encoded by the CASP3 gene which is approximately 31,6 kDa. Active caspase-3 is localised to the cytoplasm. It is involved in the TNFR1 pathway, apoptosis modulation and signalling and respiratory electron transport. This protein falls under the cysteine-aspartic acid protease family. It is a sequential activator of caspases and plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Active caspase-3 is highly expressed in lung, spleen, heart, liver and kidney. Mutations in the CASP3 gene may result in bladder urothelial carcinoma and primary effusion lymphoma. STJ97448 was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous active caspase-3 protein. |
| Applications | IF, IHC, WB |
| Dilution range | WB 1:500-1000 IHC 1:100-200 |
| Specificity | The antibody detects endogenous Active Caspase-3 protein. |
| Protein Name | Caspase-3 CASP-3 Apopain Cysteine protease CPP32 CPP-32 Protein Yama SREBP cleavage activity 1 SCA-1 Caspase-3 subunit p17 Caspase-3 subunit p12 |
| Immunogen | Recombinant Protein |
| Storage Instruction | Store at -20°C, and avoid repeat freeze-thaw cycles. |
| Note | For Research Use Only (RUO). |
| Validated Application |    |
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone ID | 50 |
| Reactivity | Avian, Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Isotype | IgGMale |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene ID | 836 |
|---|---|
| Gene Symbol | CASP3 |
| Database Links | HGNC:1504 OMIM:600636 |
| Alternative Names | Caspase-3 CASP-3 Apopain Cysteine protease CPP32 CPP-32 Protein Yama SREBP cleavage activity 1 SCA-1 Caspase-3 subunit p17 Caspase-3 subunit p12 |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. |
| Post-translational Modifications | Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. |
| Cellular Localization | Cytoplasm. |
| Tissue Specificity | Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. |
| Swiss-Prot Key | P42574_HUMAN |
](/uploads/ueditor/20200831/1-200S11505432Y.png "Anti-Active Caspase-3 antibody[STJ97448](图4)")
