Anti-Cleaved-Caspase-9 (D353) antibody[STJ90011]
Product nameAnti-Cleaved-Caspase-9 (D353) antibodyShort DescriptionRabbit polyclonal against Cleaved-Caspase-9 (D353)Descript
Product nameAnti-Cleaved-Caspase-9 (D353) antibodyShort DescriptionRabbit polyclonal against Cleaved-Caspase-9 (D353)Descript
| Product name | Anti-Cleaved-Caspase-9 (D353) antibody |
|---|---|
| Short Description | Rabbit polyclonal against Cleaved-Caspase-9 (D353) |
| Description | Caspase-9 is a protein encoded by the CASP9 gene which is approximately 46,2 kDa. Caspase-9 is localised to the cytosol, mitochondrion and nucleus. It is involved in the TNFR1 pathway, RET signalling and apoptosis modulation and signalling. Caspase-9 exist as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase-9 is ubiquitously expressed with highest expression in the heart. Mutations in the CASP9 gene may result in cerebral hypoxia and cervical cancer. STJ90011 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of fragment activated Caspase-9 protein resulting from cleavage adjacent to D353. |
| Applications | ELISA, IHC, WB |
| Dilution range | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 |
| Specificity | Cleaved-Caspase-9 (D353) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 protein resulting from cleavage adjacent to D353. |
| Protein Name | Caspase-9 CASP-9 Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 APAF-3 ICE-like apoptotic protease 6 ICE-LAP6 Caspase-9 subunit p35 Caspase-9 subunit p10 |
| Immunogen | Synthesized peptide derived from human Caspase-9 |
| Immunogen Region | 280-360 aa, C-terminal |
| Storage Instruction | Store at -20°C, and avoid repeat freeze-thaw cycles. |
| Note | For Research Use Only (RUO). |
| Validated Application | ](/uploads/ueditor/20200416/1-200416144KEO.png "Anti-Cleaved-Caspase-9 (D353) antibody[STJ90011](图1)"){kind=small} ](/uploads/ueditor/20200416/1-200416144P0962.png "Anti-Cleaved-Caspase-9 (D353) antibody[STJ90011](图2)"){kind=small} ](/uploads/ueditor/20200416/1-200416144PG35.png "Anti-Cleaved-Caspase-9 (D353) antibody[STJ90011](图3)"){kind=small} |
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Reactivity | Mouse, Rat |
| Conjugation | Unconjugated |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Isotype | IgG |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene ID | 842 |
|---|---|
| Gene Symbol | CASP9 |
| Molecular Weight | 17 kDa |
| Database Links | HGNC:1511 OMIM:602234 |
| Alternative Names | Caspase-9 CASP-9 Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 APAF-3 ICE-like apoptotic protease 6 ICE-LAP6 Caspase-9 subunit p35 Caspase-9 subunit p10 |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
| Post-translational Modifications | Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.; Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage. |
| Tissue Specificity | Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. |
| Swiss-Prot Key | P55211_HUMAN |
](/uploads/ueditor/20200825/1-200R515555U12.png "Anti-Cleaved-Caspase-9 (D353) antibody[STJ90011](图4)")
