Anti-Cleaved-Caspase-9 (D330) antibody[STJ90012]
Product nameAnti-Cleaved-Caspase-9 (D330) antibodyShort DescriptionRabbit polyclonal against Cleaved-Caspase-9 (D330)Descript
Product nameAnti-Cleaved-Caspase-9 (D330) antibodyShort DescriptionRabbit polyclonal against Cleaved-Caspase-9 (D330)Descript
| Product name | Anti-Cleaved-Caspase-9 (D330) antibody[STJ90012] |
|---|---|
| Short Description | Rabbit polyclonal against Cleaved-Caspase-9 (D330) |
| Description | Rabbit polyclonal to Cleaved-Caspase-9 (D330). |
| Applications | ELISA, IHC, WB |
| Dilution range | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:40000 |
| Specificity | Cleaved-Caspase-9 p10 (D330) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 p10 protein resulting from cleavage adjacent to D330. |
| Protein Name | Caspase-9 CASP-9 Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 APAF-3 ICE-like apoptotic protease 6 ICE-LAP6 Caspase-9 subunit p35 Caspase-9 subunit p10 |
| Immunogen | Synthesized peptide derived from human Caspase-9 p10 |
| Immunogen Region | 250-330 aa, C-terminal |
| Storage Instruction | Store at -20°C, and avoid repeat freeze-thaw cycles. |
| Note | For Research Use Only (RUO). |
| Validated Application |  |
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Isotype | IgG |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene ID | 842 |
|---|---|
| Gene Symbol | CASP9 |
| Molecular Weight | 17/37 kDa |
| Database Links | HGNC:1511 OMIM:602234 |
| Alternative Names | Caspase-9 CASP-9 Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 APAF-3 ICE-like apoptotic protease 6 ICE-LAP6 Caspase-9 subunit p35 Caspase-9 subunit p10 |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
| Post-translational Modifications | Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.; Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage. |
| Tissue Specificity | Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. |
| Swiss-Prot Key | P55211_HUMAN |
](/uploads/ueditor/20200825/1-200R5155133115.png "Anti-Cleaved-Caspase-9 (D330) antibody[STJ90012](图2)")
