Anti-Ki-67 antibody[STJ98193]
Product nameAnti-Ki-67 antibodyShort DescriptionMouse monoclonal against Ki-67DescriptionMouse monoclonal to Ki-67.Applicatio
Product nameAnti-Ki-67 antibodyShort DescriptionMouse monoclonal against Ki-67DescriptionMouse monoclonal to Ki-67.Applicatio
| Product name | Anti-Ki-67 antibody |
|---|---|
| Short Description | Mouse monoclonal against Ki-67 |
| Description | Mouse monoclonal to Ki-67. |
| Applications | ELISA, WB |
| Dilution range | WB 1:500-1:2000 ELISA 1:10000 |
| Specificity | Ki-67 Monoclonal Antibody detects endogenous levels of Ki-67 protein. |
| Protein Name | Proliferation marker protein Ki-67 Antigen identified by monoclonal antibody Ki-67 Antigen KI-67 Antigen Ki67 |
| Immunogen | Purified recombinant fragment of Ki-67 (aa3118-3256) expressed in E. Coli. |
| Immunogen Region | 3118-3256aa |
| Storage Instruction | Store at -20°C, and avoid repeat freeze-thaw cycles. |
| Note | For Research Use Only (RUO). |
| Validated Application | ](/uploads/ueditor/20200413/1-2004131533412G.png "Anti-Ki-67 antibody[STJ98193](图1)"){kind=small} |
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone ID | 9C12B2 |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Isotype | IgGMale |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 4288 |
|---|---|
| Gene Symbol | MKI67 |
| Database Links | HGNC:7107 OMIM:176741 |
| Alternative Names | Proliferation marker protein Ki-67 Antigen identified by monoclonal antibody Ki-67 Antigen KI-67 Antigen Ki67 |
| Function | Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility . Binds DNA, with a preference for supercoiled DNA and AT-rich DNA . Does not contribute to the internal structure of mitotic chromosomes . May play a role in chromatin organization . It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). |
| Post-translational Modifications | Phosphorylated. Hyperphosphorylated in mitosis . Hyperphosphorylated form does not bind DNA. |
| Cellular Localization | Chromosome Nucleus Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface . Associates with satellite DNA in G1 phase . Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes . Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix . |
| Swiss-Prot Key | P46013_HUMAN |
](/uploads/ueditor/20200825/1-200R51600494S.png "Anti-Ki-67 antibody[STJ98193](图2)")
