Anti-Phospho-Axl (Y691) antibody[STJ90939]
Product nameAnti-Phospho-Axl (Y691) antibodyShort DescriptionRabbit polyclonal against Phospho-Axl (Y691)DescriptionAxl is a
Product nameAnti-Phospho-Axl (Y691) antibodyShort DescriptionRabbit polyclonal against Phospho-Axl (Y691)DescriptionAxl is a
| Product name | Anti-Phospho-Axl (Y691) antibody[STJ90939] |
|---|---|
| Short Description | Rabbit polyclonal against Phospho-Axl (Y691) |
| Description | Axl is a protein encoded by the AXL gene which is approximately 98, 3 kDa. Axl is localised to the cell membrane. It is involved in the GPCR pathway, ERK signalling, actin nucleation by ARP-WASP complex and activation of cAMP-dependent PKA. This protein falls under the Tyro3-Axl-Mer receptor tyrosine kinase subfamily. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6. Axl is expressed in primary colon tumors but weakly expressed in normal colon tissue. Mutations in the AXL gene may result in lymphocytic choriomeningitis, femoral neuropathy and Borna disease. STJ90939 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Axl protein which only binds about Y691 when Y691 is phosphorylated. |
| Applications | ELISA, IHC-p, WB |
| Dilution range | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 |
| Specificity | Phospho-Axl (Y691) polyclonal antibody detects endogenous levels of Axl protein only when phosphorylated at Y691. |
| Protein Name | Tyrosine-Protein Kinase Receptor Ufo Axl Oncogene |
| Immunogen | Synthesized peptide derived from human Axl around the phosphorylation site of Y691. |
| Immunogen Region | 630-710 aa |
| Storage Instruction | Store at-20°C, and avoid repeat freeze-thaw cycles. |
| Note | FOR RESEARCH USE ONLY (RUO). |
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Isotype | IgG |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene ID | 558 |
|---|---|
| Gene Symbol | AXL |
| Molecular Weight | 110/97 kDa |
| Database Links | HGNC:905 OMIM:109135 |
| Alternative Names | Anti-Tyrosine-Protein Kinase Receptor Ufo antibody Anti-Axl Oncogene antibody Anti-AXL UFO antibody |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3.but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope. (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope. |
| Post-translational Modifications | Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.; Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. |
| Cellular Localization | Cell Membrane Single-Pass Type I Membrane Protein |
| Tissue Specificity | Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue. |
| Swiss-Prot Key | UFO_HUMAN |
](/uploads/ueditor/20200817/1-200QG30I2943.png "Anti-Phospho-Axl (Y691) antibody[STJ90939](图1)")
