19946275258/15301726783
| Product name | Anti-Cyclin D1 antibody【STJ92538】 |
|---|---|
| Short Description | Rabbit polyclonal against Cyclin D1 |
| Description | Cyclin D1 is a protein encoded by the CCND1 gene which is approximately 33, 7 kDa. Cyclin D1 is localised to the nucleus and cytoplasm. It is involved in CDK-mediated phosphorylation and removal of Cdc6, cyclins and cell cycle regulation. This protein falls under the highly conserved cyclin family. It is a regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of the retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition. Cyclin D1 is expressed in the nervous system, liver, skin, intestine and lung. Mutations in the CCND1 gene may result in multiple myeloma. STJ92538 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Cyclin D1 protein. |
| Applications | ELISA, IF, IHC-p, WB |
| Dilution range | IF 1:50-200 WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:40000 |
| Specificity | Cyclin D1 polyclonal antibody detects endogenous levels of Cyclin D1 protein. |
| Protein Name | G1/S-Specific Cyclin-D1 B-Cell Lymphoma 1 Protein Bcl-1 Bcl-1 Oncogene Prad1 Oncogene |
| Immunogen | Synthesized peptide derived from human Cyclin D1 around the non-phosphorylation site of T286. |
| Immunogen Region | 220-300 aa |
| Storage Instruction | Store at-20°C, and avoid repeat freeze-thaw cycles. |
| Note | FOR RESEARCH USE ONLY (RUO). |
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Isotype | IgG |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| Gene ID | 595 |
|---|---|
| Gene Symbol | CCND1 |
| Molecular Weight | 36 kDa |
| Database Links | HGNC:1582 OMIM:168461 |
| Alternative Names | Anti-G1/S-Specific Cyclin-D1 antibody Anti-B-Cell Lymphoma 1 Protein antibody Anti-Bcl-1 antibody Anti-Bcl-1 Oncogene antibody Anti-Prad1 Oncogene antibody Anti-CCND1 BCL1 PRAD1 antibody |
| Function | Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. |
| Post-translational Modifications | Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.; Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation. Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization. |
| Cellular Localization | Nucleus Cytoplasm Nucleus Membrane Cyclin D-Cdk4 Complexes Accumulate At The Nuclear Membrane And Are Then Translocated To The Nucleus Through Interaction With Kip/Cip Family Members |
| Swiss-Prot Key | CCND1_HUMAN |
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