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![Anti-Beta-tubulin antibody[STJ97037]](/uploads/allimg/20200708/1-200FQI5143Y.jpg)
![Anti-Beta-tubulin antibody[STJ97037]](/uploads/allimg/20200707/1-200FF92412300.jpg)
![Anti-Beta-tubulin antibody[STJ97037]](/uploads/allimg/20200707/1-200FF92250311.jpg)
![Anti-Beta-tubulin antibody[STJ97037]](/uploads/allimg/20200707/1-200FF92345255.png)
![Anti-Beta-tubulin antibody[STJ97037]](/uploads/allimg/20200707/1-200FF9233OQ.jpg)
![Anti-Beta-tubulin antibody[STJ97037]](/uploads/allimg/20200707/1-200FF923254G.jpg)
![Anti-Beta-tubulin antibody[STJ97037]](/uploads/allimg/20200707/1-200FF92309B3.png)
| Product name | Anti-Beta-tubulin antibody |
|---|---|
| Short Description | Mouse monoclonal against beta-tubulin |
| Description | Beta tubulin is a protein encoded by the tubb gene which is approximately 49, 7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ97037 was developed from clone M7. The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta tubulin. |
| Applications | IF, WB |
| Dilution range | WB 1:5000-10000 IF 1:100-200 |
| Specificity | The antibody detects endogenous beta-tubulin protein. |
| Protein Name | Tubulin Beta-3 Chain Tubulin Beta-4 Chain Tubulin Beta-Iii |
| Immunogen | Synthetic Peptide |
| Immunogen Region | C-term |
| Storage Instruction | Store at-20°C, and avoid repeat freeze-thaw cycles. |
| Note | FOR RESEARCH USE ONLY (RUO) |
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone ID | M7 |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Isotype | IgG1 |
| Formulation | PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide and 50% Glycerol |
| Gene ID | 10381 |
|---|---|
| Gene Symbol | TUBB3 |
| Database Links | HGNC:20772 OMIM:600638 |
| Alternative Names | Anti-Tubulin Beta-3 Chain antibody Anti-Tubulin Beta-4 Chain antibody Anti-Tubulin Beta-Iii antibody Anti-TUBB3 TUBB4 antibody |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. |
| Post-translational Modifications | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Cellular Localization | Cytoplasm Cytoskeleton Cell Projection Lamellipodium Filopodium |
| Tissue Specificity | Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. |
| Swiss-Prot Key | TBB3_HUMAN |
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